Protein knowledgebaseSequence archiveHelp pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects.Sequence clustersProtein sets from fully sequenced genomesAnnotation systemsSystems used to automatically annotate proteins with high accuracy:Supporting dataSelect one of the options below to target your search:You are using a version of browser that may not display all the features of this website. Please consider upgrading . Integrin-linked protein kinaseIlkMus musculus (Mouse)-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveliFunctioniReceptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B.Manual assertion inferred from sequence similarity toiCatalytic activityiATP + a protein = ADP + a phosphoprotein.Enzyme regulationiStimulated rapidly but transiently by both cell fibronectin interactions, as well as by insulin, in a PI3-K-dependent manner, likely via the binding of PtdIns(3,4,5)P3 with a PH-like domain of ILK. The protein kinase activity is stimulated by LIMD2.Manual assertion inferred from sequence similarity toiSitesFeature keyPosition(s)DescriptionActionsGraphical viewLengthBinding siteiATPManual assertion according to rulesi1RegionsFeature keyPosition(s)DescriptionActionsGraphical viewLengthNucleotide bindingiATPManual assertion according to rulesi9GO - Molecular functioniInferred from sequence orthologyiGO - Biological processiInferred from direct assayiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from sequence orthologyiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiInferred from mutant phenotypeiKeywordsiMolecular function, , Ligand, Enzyme and pathway databasesBRENDAi 3474. Reactomei Localization of the PINCH-ILK-PARVIN complex to focal adhesions. Names & TaxonomyiProtein namesiRecommended name:Integrin-linked protein kinase (EC:)Gene namesiName:OrganismiTaxonomic identifieri
[]Taxonomic lineagei >
Proteomesi Componenti: Chromosome 7 Organism-specific databasesMGIi Ilk. Subcellular locationi >
Manual assertion based on experiment ini"Affixin activates Rac1 via betaPIX in C2C12 myoblast.", , , , , , ,
[] [] []Cited for: SUBCELLULAR LOCATION."Kindlin-2 controls bidirectional signaling of integrins.", , , , , ,
[] [] []Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FERMT2. ;
Manual assertion inferred from sequence similarity toi >
Manual assertion inferred from sequence similarity toi >
Manual assertion based on experiment ini"Affixin activates Rac1 via betaPIX in C2C12 myoblast.", , , , , , ,
[] [] []Cited for: SUBCELLULAR LOCATION.GO - Cellular componentiInferred from direct assayiInferred from direct assayiKeywords - Cellular componenti, , , , PTM / ProcessingiMolecule processingFeature keyPosition(s)DescriptionActionsGraphical viewLengthChainiPRO_Integrin-linked protein kinase 452Amino acid modificationsFeature keyPosition(s)DescriptionActionsGraphical viewLengthModified residueiN-acetylmethionineManual assertion inferred from sequence similarity toi1Modified residueiPhosphoserineManual assertion inferred from sequence similarity toi1Modified residueiN6-acetyllysineManual assertion inferred from combination of experimental and computational evidencei"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.", , , , , , , , , , ,
[] [] []Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-426, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].1Post-translational modificationiAutophosphorylated on serine residues.Keywords - PTMi, Proteomic databasesEPDi MaxQBi PaxDbi PeptideAtlasi PRIDEi PTM databasesiPTMneti PhosphoSitePlusi SwissPalmi ExpressioniTissue specificityiHighly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis.Gene expression databasesBgeei CleanExi ExpressionAtlasi baseline and differential. Genevisiblei MM. InteractioniSubunit structureiInteracts with FERMT2 (PubMed:). Interacts with the cytoplasmic domain of ITGB1 (By similarity). Could also interact with integrin ITGB2, ITGB3 and/or ITGB5 (By similarity). Interacts (via ANK repeats) with LIMS1 and LIMS2 (By similarity). Interacts with PARVA and PARVB; these compete for the same binding site (By similarity). Interacts probably also with TGFB1I1 (PubMed:). Interacts (via ANK repeats) with EPHA1 (via SAM domain); stimulated by EFNA1 but independent of the kinase activity of EPHA1 (By similarity). Interacts with LIMD2; leading to activate the protein kinase activity (By similarity).Manual assertion inferred from sequence similarity toiManual assertion based on experiment ini"Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex.", , , , , , , ,
[] [] []Cited for: INTERACTION WITH TGFB1I1."Kindlin-2 controls bidirectional signaling of integrins.", , , , , ,
[] [] []Cited for: SUBCELLULAR LOCATION, INTERACTION WITH FERMT2.Binary interactionsiGO - Molecular functioniProtein-protein interaction databasesBioGridi 6 interactors.DIPi IntActi 47 interactors.MINTi STRINGi Structurei3D structure databasesProteinModelPortali SMRi ModBaseiMobiDBiFamily & DomainsiDomains and RepeatsFeature keyPosition(s)DescriptionActionsGraphical viewLengthRepeatiANK 1 29RepeatiANK 2 33RepeatiANK 3 33RepeatiANK 4 33RepeatiANK 5 45DomainiProtein kinaseManual assertion according to rulesi 254RegionFeature keyPosition(s)DescriptionActionsGraphical viewLengthRegioniInteraction with LIMS1 107RegioniPH- mediates interaction with TGFB1I1Manual assertion based on experiment ini"Oligomerizing potential of a focal adhesion LIM protein Hic-5 organizing a nuclear-cytoplasmic shuttling complex.", , , , , , , ,
[] [] []Cited for: INTERACTION WITH TGFB1I1. 33DomainiA PH-like domain is involved in phosphatidylinositol phosphate binding.Manual assertion inferred from sequence similarity toiSequence similaritiesiBelongs to the . .Contains 5 s.Manual assertion according to rulesiContains 1 .Manual assertion according to rulesiKeywords - Domaini, Phylogenomic databaseseggNOGi Eukaryota.
LUCA. GeneTreei HOGENOMi HOVERGENi InParanoidi KOi OMAi OrthoDBi PhylomeDBi TreeFami Family and domain databasesGene3Di 2 hits. InterProi Ankyrin_rpt.
Ankyrin_rpt-contain_dom.
Kinase-like_dom.
Prot_kinase_dom.
Ser-Thr/Tyr_kinase_cat_dom.
[]Pfami Ank_2. 2 hits.
Pkinase_Tyr. 1 hit.
[]SMARTi ANK. 3 hits.
[]SUPFAMi SSF48403. 1 hit.
SSF56112. 1 hit. PROSITEi ANK_REP_REGION. 1 hit.
ANK_REPEAT. 3 hits.
PROTEIN_KINASE_DOM. 1 hit.
[]SequenceiSequence statusi: Complete.O55222-1 []
50MDDIFTQCRE GNAVAVRLWL DNTENDLNQG DDHGFSPLHW ACREGRSAVV
100EMLIMRGARI NVMNRGDDTP LHLAASHGHR DIVQKLLQYK ADINAVNEHG
150NVPLHYACFW GQDQVAEDLV ANGALVSICN KYGEMPVDKA KAPLRELLRE
200RAEKMGQNLN RIPYKDTFWK GTTRTRPRNG TLNKHSGIDF KQLNFLAKLN
250ENHSGELWKG RWQGNDIVVK VLKVRDWSTR KSRDFNEECP RLRIFSHPNV
300LPVLGACQAP PAPHPTLITH WMPYGSLYNV LHEGTNFVVD QSQAVKFALD
350MARGMAFLHT LEPLIPRHAL NSRSVMIDED MTARISMADV KFSFQCPGRM
400YAPAWVAPEA LQKKPEDTNR RSADMWSFAV LLWELVTREV PFADLSNMEI
450GMKVALEGLR PTIPPGISPH VCKLMKICMN EDPAKRPKFD MIVPILEKMQ DK
45251,373October 31, 2006 - v2Checksum:iF43A7BLASTProtParamProtScaleCompute pI/MWPeptideMassPeptideCutterExperimental InfoFeature keyPosition(s)DescriptionActionsGraphical viewLengthSequence conflictiI → T in
(PubMed:).1Sequence conflictiI → V in
(PubMed:).1Sequence databasesSelect the link destinations:EMBLiGenBankiDDBJi mRNA. Translation: . mRNA. Translation: .CCDSi RefSeqi
UniGenei Genome annotation databasesEnsembli; ; . ; ; . GeneIDi KEGGi UCSCi mouse. Cross-referencesiSequence databasesSelect the link destinations:EMBLiGenBankiDDBJi mRNA. Translation: . mRNA. Translation: .CCDSi RefSeqi
UniGenei 3D structure databasesProteinModelPortali SMRi ModBaseiMobiDBiProtein-protein interaction databasesBioGridi 6 interactors.DIPi IntActi 47 interactors.MINTi STRINGi PTM databasesiPTMneti PhosphoSitePlusi SwissPalmi Proteomic databasesEPDi MaxQBi PaxDbi PeptideAtlasi PRIDEi Protocols and materials databasesStructural Biology KnowledgebaseGenome annotation databasesEnsembli; ; . ; ; . GeneIDi KEGGi UCSCi mouse. Organism-specific databasesCTDi MGIi Ilk. Phylogenomic databaseseggNOGi Eukaryota.
LUCA. GeneTreei HOGENOMi HOVERGENi InParanoidi KOi OMAi OrthoDBi PhylomeDBi TreeFami Enzyme and pathway databasesBRENDAi 3474. Reactomei Localization of the PINCH-ILK-PARVIN complex to focal adhesions. Miscellaneous databasesPROi SOURCEiGene expression databasesBgeei CleanExi ExpressionAtlasi baseline and differential. Genevisiblei MM. Family and domain databasesGene3Di 2 hits. InterProi Ankyrin_rpt.
Ankyrin_rpt-contain_dom.
Kinase-like_dom.
Prot_kinase_dom.
Ser-Thr/Tyr_kinase_cat_dom.
[]Pfami Ank_2. 2 hits.
Pkinase_Tyr. 1 hit.
[]SMARTi ANK. 3 hits.
[]SUPFAMi SSF48403. 1 hit.
SSF56112. 1 hit. PROSITEi ANK_REP_REGION. 1 hit.
ANK_REPEAT. 3 hits.
PROTEIN_KINASE_DOM. 1 hit.
[]ProtoNetiMiscellaneousiKeywords - Technical termi, Documents
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
Human and mouse protein kinases: classification and index
Index of protein domains and families
Similar proteinsiLinks to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.}