中国科学院上海应用物理研究所物理生物学实验室
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Publications　
and C. Fan, DNA reaction networks: providing a panoramic
view. Nature Chem. 8, 738 (2016).
NPG Asia Mater.& 8, e306 (2016).
Gao, A., Lu,
N., Wang, Y., Dai, P., Li, T., Gao, X., Wang, Y. and Fan, C.
(2012) Enhanced sensing of Nucleic Acids with Silicon
Nanowire Felid Effect Transistor Biosensors. Nano Lett.,
and Lee, S.-T.
Y., Su, S., Xu, T., Zhong, Y., Zapien, A., Li, J., Fan, C. and
Lee, S.-T. (2011) Silicon Nanowires-Based Highly-Efficient
SERS-Active Platform for Ultrasensitive DNA Detection.
Nano Today, 6, 122-130.
7.&&&Peng,
C., Jiang, B., Liu, Q., Guo, Z., Xu, Z., Huang, Q., Xu, H.,
Tai, R. and Fan, C. (2011) Graphene-templated formation of
two-dimensional lepidocrocite nanostructures for
high-efficiency catalytic degradation of phenols. Energy
Environ. Sci.,
D., Song, S. and Fan, C. (2010) Target-responsive structures
for nucleic acid sensors. Acc. Chem. Res.,
Zhang, Z., Wang,
Y., Fan, C., Li, C., Li, Y., Qian, L., Fu, Y., Shi, Y., Hu,
J. and He, L. (2010) Asymmetric DNA origami for spatially
addressable and index-free solution-phase DNA chips. Adv.
12.&Hu, M., Yan,
J., He, Y., Lu, H., Weng, L., Song, S., Fan, C.
and Wang, L. (2010) Ultrasensitive, multiplexed detection of
cancer biomarkers directly in serum by using a quantum dot
based microfluidic protein chip. ACS Nano,
Zhang, F., Zhang, F., Su, H., Li, H.,
Zhang, Y., Hu, J. (2010) Mechanical Manipulation Assisted
Self-Assembly To Achieve Defect Repair and Guided Epitaxial
Growth of Individual Peptide Nanofilaments. ACS Nano, 4,
Su, Y., Hu, M.,
Fan, C., He, Y., Li, Q., Li, W., Wang, L., Shen, P. and
Huang, Q. (2010) The cytotoxicity of CdTe quantum dots and
the relative contributions from released cadmium ions and
nanoparticle properties. Biomaterials,
17. Li, J.,
Zhu, Y., Li, W., Zhang, X., Peng, Y., Huang, Q. (2010)
Nanodiamonds as intracellular transporters of
chemotherapeutic drug. Biomaterials, 31,
23. Yan, J.,
Song, S., Li, B., Huang, Q., Zhang, Q., Zhang, H. and Fan,
C. (2010) An on-nanoparticle rolling circle amplification
platform for ultrasensitive protein detection in biological
fluids. Small,
Y., Kang, Z., Li, Q., Tsang, C.H.A., Fan, C. and Lee, S.-T. (2009)
Ultrastable, Highly Fluorescent, and Water-Dispersed
Silicon-Based Nanospheres as Cellular Probes. Angew.
Chem. Int. Ed., 48, 128-132.
& He, Y., Su, Y.,
Yang, X., Kang, Z., Xu, T., Zhang, R., Fan, C. and Lee,
S.-T. (2009) Photo and pH Stable, Highly-Luminescent Silicon Nanospheres and Their Bioconjugates for Immunofluorescent
Cell Imaging. J. Am. Chem. Soc., 131,
5.&& Wang, C., Lu, H., Wang, Z., Xiu, P., Zhou, B., Zuo, G., Wan,
R., Hu, J. and Fang, H. (2009)
Stable Liquid Water Droplet on a Water Monolayer Formed at
Room Temperature on Ionic Model Substrates. Phys. Rev.
Lett., 103, 137801.
Mi, L., Wen, Y., Pan, D., Wang, Y., Fan,
C. and Hu, J. (2009) Modulation of DNA Polymerases with Gold
Nanoparticles and their Applications in Hot-Start PCR.
Small, 5, .
Y., He, Y., Lu, H., Sai, L., Li, Q., Li, W., Wang, L., Shen, P., Huang,
Q. and Fan, C. (2009) The cytotoxicity of cadmium based,
aqueous phase-synthesized, quantum dots and its modulation
by surface coating. Biomaterials, 30, 19-25.
& Luo, C., Zuo, X.,
Wang, L., Wang, E., Song, S., Wang, J., Wang, J., Fan, C.
and Cao, Y. (2008) Flexible Carbon Nanotube-Polymer
Composite Films with High Conductivity and
Superhydrophobicity Made by Solution Process. Nano Lett.,
& He, Y., Lu, H.,
Sai, L., Su, Y., Hu, M., Fan, C., Huang, W. and Wang, L.
(2008) Microwave synthesis of water-dispersed CdTe/CdS/ZnS
core-shell-shell quantum dots with excellent photostability
and biocompatibility. Adv. Mater., 20,
& Liu, G., Wan, Y.,
Gau, V., Zhang, J., Wang, L., Song, S. and Fan, C. (2008) An
enzyme-based E-DNA sensor for sequence-specific detection of
femtomolar DNA targets. J. Am. Chem. Soc., 130,
& Li, J., Song, S.,
Liu, X., wang, L., Pan, D., Huang, Q., Zhao, Y. and Fan, C.
(2008) Enzyme-based multi-component optical nanoprobes for
sequence-specific detection of DNA hybridization. Adv.
Mater., 20, 479-500.
& Zhang, J., Wang,
L., Pan, D., Song, S., Boyer, F.Y., Zhang, H. and Fan, C.
(2008) Visual Cocaine Detection with Gold Nanoparticles and
Rationally Engineered Aptamer Structures. Small, 4,
1.Gong, X., Li,
J.,& Lu, H., Wan, R., Li, J., Hu, J., Fang, H.
A charge driven
molecular water pump,
Nature Nanotechnology, 2, 709-712.
Zhang, J., Song,
S., Wang, L., Pan, D. and Fan, C. (2007) A gold
nanoparticle-based chronocoulometric DNA sensor for
amplified detection of DNA. Nature Protocols, 2,
Wang, J., Wang,
L., Liu, X., Liang, Z., Song, S., Li, W., Li, G. and Fan, C.
(2007) A gold nanoparticle-based aptamer target binding
readout for ATP assay. Adv. Mater., 19,
& Zuo, X., Song,
S., Zhang, J., Pan, D., Wang, L. and Fan, C. (2007) A
target-responsive electrochemical aptamer switch (TREAS) for
reagentless detection of nanomolar ATP. J. Am. Chem. Soc.,
6.Liu, X., Tang,
Y., Wang, L., Zhang, J., Song, S., Fan, C. and Wang, S.
(2007) Optical detection of mercury (II) in aqueous
solutions using conjugated polymers and label-free
oligonucleotides. Adv. Mater., 19, .
Chen, X., Wang,
Y., Liu, Q., Zhang, Z., Fan, C. and He, L. (2006)
Construction of molecular logic gates with a DNA-cleaving
deoxyribozyme. Angew. Chem. Int. Ed., 45,
F., Du, H., Zhang, Z., Ji, L., Li, H., Tang, L., Wang, H.,
Fan, C., Xu, H., Zhang, Y. et al.
(2006) Epitaxial
Growth of Peptide Nanofilaments on Inorganic Surfaces:
Effects of Interfacial Hydrophobicity/Hydrophilicity.
Angew. Chem. Int. Ed., 45, .
Zhang, J., Song,
S., Zhang, L., Wang, L., Wu, H., Pan, D. and Fan, C. (2006)
Sequence-specific detection of femtomolar DNA via a
chronocouloumetric DNA sensor (CDS): Effects of
nanoparticle-mediated amplification and nanoscale control of
DNA assembly at electrodes. J. Am. Chem. Soc., 128,
5.&&Ouyang, Z., Tan, L., Liu, M., Judge, O. S.,
Zhang, X., Li, H., Hu, J., Patten, T. E. and Liu, G. (2006)
A Simple Miniaturization Protocol to Produce Multicomponent
Micro-and Nanostructures. Small, 2, 884-887.
Li, H., Huang,
J., Lv, J., An, H., Zhang, X., Zhang, Z., Fan, C. and Hu, J.
(2005) Nanoparticle PCR: Nanogold-assisted PCR with enhanced
specificity. Angew. Chem. Int. Ed., 44, 5100.
Lu, J., Li, H.,
An, H., Wang, G., Wang, Y., Li, M., Zhang, Y., &Hu, J.
(2004) Positioning isolation and biochemical analysis of
single DNA molecules based on nanomanipulation and
single-molecule PCR, J. Am. Chen. Soc., 126,
Fan, C., Plaxco,
K.W. and Heeger, A.J. (2005) Biosensors based on
binding-modulated donor-acceptor distances. Trends in
Biotechnology, 23, 186-192.
Zhang, Y., Gao, H., Li, M. and Hartmann, U. (2002)
Artificial DNA patterns by
mechanical nanomanipulation,
Nano Lett., 2, 55-57.
Xu, H., Wu, H.,
Huang, F., Song, S., Li, W., Cao, Y. and Fan, C. (2005)
Magnetically assisted DNA assays: High selectivity using
conjugated polymers for amplified fluorescent transduction.
Nucleic Acids Res., 33, e83.Study of O-Phosphorylation Sites in Proteins Involved in Photosynthesis-Related Processes in Synechocystis sp. Strain PCC 6803: Application of the SRM Approach -
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|Study of O-Phosphorylation Sites in ...
O-Phosphorylation has been shown in photosynthesis-related proteins in a cyanobacterium Synechocystis sp. strain PCC 6803 (thereafter Synechocystis 6803), suggesting that phosphorylation of S, T, and Y residues might be important in photosynthesis-related processes. Investigation of biological roles of these phosphorylation events requires confident knowledge of the phosphorylated sites and prospects for their individual assessment. We performed phosphoproteomic analysis of Synechocystis 6803 using TiO2 enrichment of the phosphopeptides, followed by LC–MS/MS, and discovered 367 phosphorylation sites in 190 proteins participating in various cellular functions. Furthermore, we focused on the large group of phosphoproteins that are involved in light harvesting, photosynthesis-driven electron flow, photoprotection, and CO2 fixation. The SRM approach was applied to verify/improve assignments of phosphorylation sites in these proteins and to investigate possibilities for analysis of phosphopeptide isomers. The ...
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The successful applicability of gene therapy approaches will heavily rely on the development of efficient and safe nonviral gene delivery vectors, for example, cell-penetrating peptides (CPPs). CPPs can condense oligonucleotides and plasmid DNA (pDNA) into nanoparticles, thus allowing the t ...
Dendrimers feature a defined number of terminal groups that may bind RNA or be functionalized with bioactive molecules. These competing uses of terminal groups may create an impasse if the requisite density of ligands depletes the number of terminal groups for binding sufficient RNA, or vic ...
The development of clay bionanocomposites requires processing routes with nanostructural control. Moreover, moisture durability is a concern with water-soluble biopolymers. Here, oriented bionanocomposite coatings with strong in-plane orientation of clay platelets are for the first time pre ...
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